BROWN-BRENN MODIFICATION OF GRAM STAIN
BROWN BRENN STAINING It is a modified gram staining technique used in pathology for the demonstration of bacteria in tissue samples. The principle used here is similar to that of gram staining. The same cell wall theory of bacteria is utilized here. Reagents: 1% Crystal violet Lugol's Iodine Acetone 0.25%Basic Fuchsin Picric Acid-acetone Procedure: 1. Add crystal violet into the tissue section and allow it to stand for 1 minute 2. Wash and add Lugol's Iodine for 1 minute. 3. Wash again and add acetone over the section until no color turns off. 4. Wash the slide and pour basic fuchsin and stai...
